Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1962; 07(02): 295-303DOI: 10.1055/s-0038-1655477 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Peptidase Activity in Human Platelets Walter Kocholaty 1 Biochemistry Division, US Army Medical Research Laboratory, Fort Knox, Kentucky , with technical assistance of Miss S. M. Horn› Author AffiliationsRecommend Article Abstract PDF Download Buy Article Summary Normal human platelets contain a variety of di- and tripeptidases. The hydrolytic action of platelet extracts on glycylglycine, glycyl-L-leucine and L-leucylglycine was investigated under a variety of conditions. The pH optima, heavy metal activation and some other properties of these enzymes were described and compared with that of serum and the other formed elements of the blood. PDF (1445 kb) References References 1 Fleisher G. A, Butt H. R, Huizenga K. A. Serum peptidases in hepatic disease. Ann. N. Y. Acad. Sci 1958; 75: 363 2 Fleisher G. A, Butt H. R. Peptidases in human serum; a comparison of activities between normal persons and patients with liver diseases and other pathologic conditions. J. clin. Invest 1953; 32: 674 3 Fleisher G. A. Peptidases in human blood, I. The hydrolysis of glycylglycine and glycyl-L-leucine by normal serum. J. biol. Chem 1953; 205: 925 4 Fleisher G. A. Peptidases in human blood, II. The hydrolysis of additional peptides by normal serum. J. biol. Chem 1954; 206: 637 5 Adams E, McFadden M, Smith E. L. Peptidase of erythrocytes, I. Distribution in man and other species. J. biol. Chem 1952; 198: 663 6 Adams E, Smith E. L. Peptidases of erythrocytes, II. Isolation and properties of prolidase. J. biol. Chem 1952; 198: 671 7 Adams E, Davis N. C, Smith E. L. Peptidases of erythrocytes, III. Tripeptidase. J. biol. Chem 1952; 199: 845 8 Tsuboi K. K, Penefsky Z. J, Hudson P. B. Enzymes of the human erythrocyte, III. Tripeptidase, purification and specific properties. Arch. Biochem 1957; 68: 54 9 Fleisher G. A. Peptidases in human leukocytes. Leukocytic functions. Ann. N. Y. Acad. Sci 1955; 59: 1012 10 Fleisher G. A. Peptidases in human blood, IV. The hydrolysis of glycyl-L-leucine and other dipeptides by leukocytes. Arch. Biochem 1956; 61: 119 11 Fleisher G. A. In Peptidases of Human Leukozytes. Methods in Medical Research. Vol. VII. Warren J. V. Ed The Year Book Publishers, Inc.; Chicago: 1958: 150 12 Kocholaty W, Horn S. M. Peptidases in mammalian platelets I and II USAMRL Report No. 377, 1959, and USAMRL Report No. 451, 1960, Fort Knox, Kentucky 13 Moore S, Stein W. H. Photometric ninhydrin method for use in the chromatography of amino acids. J. biol. Chem 1948; 176: 367 14 Schwartz T. B, Engel F. L. A photometric ninhydrin method for the measurement of proteolysis. J. biol. Chem 1950; 184: 197 15 Smith E. L. Studies on dipeptidases, II. Some properties of the glycyl-L-leucine dipeptidase of animal tissues. J. biol. Chem 1948; 176: 9 16 Smith E. L. Studies on dipeptidases, III. Hydrolysis of methylated peptides; the role of cobalt in the action of glycylglycine dipeptidase. J. biol. Chem 1948; 176: 21 17 Husefeldt E. Proteolytische Enzyme in den Leukozyten des Menschen. Z. physiol. Chemie 1931; 194: 137 18 Smith E. L. Dipeptidases. Methods in Enzymology II. Colowick S. P, Kaplan N. O. (Eds.) Academic Press, Inc.; New York: 1955: 93 19 Neuman R. E, Smith E. L. Synthesis of proline and hydroxyproline peptides; their cleavage by prolinase. J. biol. Chem 193: 97 1951; 20 Smith E. L. Aminopeptidases. Methods in Enzymology II. Colowick S. P, Kaplan N. O. (Eds.) Academic Press, Inc.; New York: 1955: 83 21 Fruton J. S, Smith V. A, Driscoll P. A. On the proteolytic enzymes of animal tissues, VII. A peptidase of calf thymus. J. biol. Chem 1948; 173: 457 22 Ellis D, Fruton J. S. On the proteolytic enzymes of animal tissues, IX. Calf thymus tripeptidase. J. biol. Chem 1951; 191: 153 23 Bricas E, Fromageot C. Naturally occurring peptides. Advanc. Protein Chem 1953; 8: 1
